PathHunter® Cell-Based Kinase Screening Services

Characterize compounds in a whole cell context

The PathHunter® Cell-Based Kinase Screening and Profiling Service leverages DiscoveRx's proprietary Enzyme Fragment Complementation (EFC) Assay Technology in a whole cell platform enabling customers to interrogate and characterize compounds in a cellular context.  This robust cell assay platform provides customers with key potency and selectivity data against an expanding portfolio of tyrosine kinases and a powerful solution to support identification and elucidation of a diverse range of chemical and biological entities and mechanisms of action including non-ATP competitive inhibitors, ligand binding inhibitors and dimerization inhibitors.  This service affords researchers flexible options and multiple screening parameters including dose response (EC50) and single concentration screens in agonist or antagonist mode against any number of compounds.


  Schematic of the PathHunter® Tyrosine Kinase Assay Principle


Panel Highlights and Benefits

  • Cell-based functional assay
  • Elucidate target pathways in a physiological relevant context
  • Identify agonists & antagonists
  • High-quality, quantitative, reproducible data
  • Flexible screening parameters & assay modes
  • Detection of multiple inhibitor types

Receptor and Cytosolic Tyrosine Kinase Assays

We currently have 18 receptor tyrosine and 3 cytosolic tyrosine kinase targets available for profiling, screening and selectivity studies. We are continuing to develop additional targets in order to provide the most comprehensive panel.  Please contact us if your desired target isn't available - we excel in Custom Assay Development.
 

Receptor Tyrosine Kinase Assays

KINOMEscan Gene Symbol Target Partner Protein Cell Line Details
MET C-MET   U2OS click here
DDR1 DDR1   U2OS click here
DDR2 DDR2   U2OS click here
EPHB4 EphB4   U2OS click here
EGFR ErbB1   U2OS click here
ERBB2 ErbB2/ErbB3 Grb2 U2OS click here
ERBB4 ErbB4 SHC1 U2OS click here
FGFR4 FGFR4 PLCG2 U2OS click here
FLT3 Flt3 P85 U2OS click here
IGF1R IGFR1 SHC1 1321N1 click here
INSR INSR PLCG1 U2OS click here
PDGFRB PDGFRb PLCG1 U2OS click here
TRKA TrkA SHC1 U2OS click here
  TrkA-P75 SHC1 U2OS click here
TRKB TrkB SHC1 U2OS click here
  TrkB-P75 SHC1 U2OS click here
TRKC TrkC SHC1 U2OS click here
  TrkC-P75 SHC1 U2OS click here

 

Cytosolic Tyrosine Kinase Assays

KINOMEscan Gene Symbol Target Partner Protein Cell Line Details
JAK1(JH1domain-catalytic) JAK1 PRLR U2OS click here
JAK2(JH1domain-catalytic) JAK2 PRLR U2OS click here
JAK2(JH1domain-catalytic) JAK2 GHR   Coming Soon!


Click here to request a quote.  Note that links on this page will take you to the DiscoveRx website.