scanLIPID Kinase Assay Panel

20 assays - the largest lipid kinase assay panel available

The largest commercial collection of human lipid kinase assays available, scanLIPID contains a panel of PI3K, PI4K, PIP plus clinically relevant mutants and represents an ideal solution, alone or paired with additional relevant kinases, to explore this target class. 

scanLIPID is a focused panel for all stages of drug discovery and development, from high throughput HTS campaigns associated with lead discovery & hit identification to lower throughput applications including benchmarking small number of compounds, lead optimization and preclinical applications.

Panel Highlights and Benefits

  • Panel of 20 high value lipid kinase assays
  • High-quality reproducible data
  • Rapid turnaround time
  • Broad dynamic range: detect compounds with Kds <100 pM to >10 mM
  • Flexible - Standard and custom panels 
KINOMEscan Kinase Dendrogram

Red circles (above) represent the kinases currently available in the KINOMEscan scanLIPID assay panel (Mutant forms of lipid kinase assays not shown).

Assay Sensitivity

Assay Sensitivity and Range

Binding constant (Kd) determinations for the indicated compounds against LOK demonstrate the broad range (> 5 logs) of interaction affinities quantitatively measured using the KINOMEscan assay platform.  Unlike for traditional assay formats, KINOMEscan assays are performed at exceedingly low kinase concentrations (<0.1 nM), which enables the measurement of accurate Kd values in the pM range.  The KINOMEscan assay platform is not fluorescence-based and therefore avoids any “interference” problems at high compound concentrations, and the upper limit of Kd detection is therefore limited only by a test compound’s solubility.[click graph to enlarge].

Assay Quality

Z' Factor Analysis - one metric of assessing assay quality

Average Z' values and standard deviations were calculated for each kinase based on fourteen control wells per experiment in over 135 independent experiments spanning a period of sixteen months. Average Z' = 0.68 [click graph to enlarge].

 

        Data Consistency


Profiling of the indicated compounds at 10uM in fourteen independent experiments against 442 kinases over a one year period. Correlation analysis was performed in a pair-wise comparison to calculate the correlation coefficient. The correlation coefficients range from 0.91 to 0.97 with an average of 0.95 [click graph to enlarge].